Alexa Fluor 488 is a very good choice for flow cytometry. has a great extinction coefficient, its quantum yield is too weak to make mRaspberry a good choice for

The extinction coefficient (ε) of Alexa Fluor 430 isEasily find your required extinction coefficient (ε) with AAT Bioquest's Extinction Coefficient Database. Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak.

The chemical environment for example pH also affects the quantum yield. The molar extinction coefficient (ε) for Alexa Fluor 488 is 73,000 cm-1M-1. To request additional information and datasets for Alexa Fluor 488, please contact info@aatbio.com. where 71,000 cm-1M is the approximate molar extinction coefficient of the Alexa Fluor® 488 dye at 494 nm. For IgGs, we find that labeling with 4–9 moles of Alexa Fluor® 488 dye per mole of antibody is optimal. Storing and Handling the Conjugates Store the labeled protein—which will be in PBS, pH 7.2, containing ~2 mM sodium azide—at Fluorophore label: Alexa Fluor® 488 dye Reactive group: maleimide Reactivity: thiol groups on proteins and ligands, oligonucleotide thiophosphates Ex/Em of the conjugate: 493/516 nm Extinction coefficient: 72,000 cm-1M-1 Spectrally similar dyes: Fluorescein (FITC), Cy®2 Molecular weight: 720.66 Typical Conjugation Reaction Extinction Coefficient ‡ Alexa Fluor 350: 346: 442: Blue: 19,000: Alexa Fluor 405: 402: 421: Blue: 35,000: Alexa Fluor 430: 434: 539: Yellow-green: 15,000: Alexa Fluor 488: 495: 519: Green: 73,000: Alexa Fluor 514: 518: 540: Green: 80,000: Alexa Fluor 532: 531: 554: Yellow: 81,000: Alexa Fluor 546: 556: 573: Orange: 112,000: Alexa Fluor 555: 555: 565: Orange: 155,000: Alexa Fluor 568: 578: 603: Red-orange: 88,000: Alexa Fluor 594: 590: 617 Extinction coefficient** Alexa Fluor 350: 346: 442: Blue: 19,000: Alexa Fluor 405: 401: 421: Blue: 34,000: Alexa Fluor 430: 433: 541: Green/Yellow: 16,000: Alexa Fluor 488: 496: 519: Green: 71,000: Alexa Fluor 532: 532: 553: Yellow: 81,000: Alexa Fluor 546: 556: 573: Orange: 104,000: Alexa Fluor 555: 555: 565: Orange: 150,000: Alexa Fluor 568: 578: 603: Orange/Red: 91,000: Alexa Fluor 594: 590: 617: Red: 73,000: Alexa Fluor 610: 612: 628: Red Invitrogen Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 488 dye is pH-insensitive over a wide molar range.

Af 488 extinction coefficient

516 green (dark). Alexa fluor 488. 494. 517 green (light). Approximate absorption (Abs) and fluorescence emission (Em) maxima for conjugates, in nm. † Mixed isomers. ‡ The APEX Alexa Fluor 488 Antibody Labeling av M Matson Dzebo · 2014 — AF-16 is a peptide derived from the natural protein Antisecretory Factor (AF), pathlength that the light travels in the sample, l, and the extinction coefficient of Cellular uptake of AF-16 (Alexa Fluor 488-labeled) in live wild type CHO-K1 and.

Two-photon absorption in gapped bilayer graphene with a tunable chemical potential2016Ingår i: Journal of Physics: Condensed Matter, ISSN 0953-8984, 488-493Artikel i tidskrift (Refereegranskat).

203,000 = molar extinction coefficient ( ε) of a IgG at 280 nm [cm-1. M-1] 90,000 = molar extinction coefficient (ε) of the Atto 488 dye at 501 nm [cm-1. M-1] 0.101 = correction factor due to the fluorophore’s absorbance at 280 nm . Storage / Stability . For …

Correction factor used in calculation of The Alexa Fluor family of fluorescent dyes is a series of dyes invented by Molecular Probes, now a part of Thermo Fisher Scientific, and sold under the Invitrogen brand name. Alexa Fluor dyes are frequently used as cell and tissue labels in fluorescence microscopy and cell biology. Alexa Fluor dyes can be conjugated directly to primary antibodies or to secondary antibodies to amplify signal and sensitivity or other … AFDye™ 488 is a bright, highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. This probe is water-soluble and its fluorescence is pH independent over a wide pH range.

The extinction coefficient (ε) of Alexa Fluor 430 isEasily find your required extinction coefficient (ε) with AAT Bioquest's Extinction Coefficient Database. Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak.

(1972) report that female M. minimus lagochi/us Matschie, 1899 in Sarawak was pregnant On Lombok I. on 19 October the ratio of adult males to adult females was I:1.9. Sixty-four ofthe 86 L371, L470. L315, L370, L47I, L488 Biogeography of mammals in SE Asia, estimates of rates of colonisation, extinction. The absorption coefficient of tissue is strongly wavelength dependent.

Défauts entraînant un arc irrégulier ou son extinction Défaut Cause possible Les 7 1 1366 488-001 | Sidoplát Side plate Seitenblech Coté (liten).
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Fluor 555 dye was similar to the Cy3 dye, and the Alexa Fluor 647 dye was Extinction coefficients for all unconjugated dyes in methanol length of 488 nm.

For details of coupling see our recommended labeling procedure at www.atto-tec.com - Support - Downloads - General Procedures. Product Information: ATTO 488 300 400 500 600 700 800 900 1000 wavelength, nm absorption fluorescence l abs = 500 nm e max = 9.0 x 104 M-1 cm-1 l fl = 520 nm h fl = 80 % t fl Extinction coefficient: 40,000 Molecular weight: ~429 Excitation laser line: 405 nm Replaces: Pacific Green®, BD Horizon™ V500, Krome Orange™ CF®440 Abs/Em Maxima: 440/515 nm Extinction coefficient: 40,000 Molecular weight: ~716 Excitation laser line: 405 nm Replaces: Alexa Fluor® 430 Extinction Coefficient (ε) (M-1cm ) Fluorescence Quantum Yield (φ) Fluorescence Life Time (τ) (ns) HiLyte Fluor™ 405 404 428 35,000 0.54 Not available HiLyte Fluor™ 488 497 525 70,000 0.91 4.1 HiLyte Fluor™ 555 550 566 150,000 0.10 0.3 HiLyte Fluor™ 594 593 616 80,000 0.90 4.2 HiLyte Fluor™ 647 649 672 250,000 0.33 1.0 Extinction Coefficient (ε) 75000 M-1 cm-1: Quantum Yield (φ) 0.92: Closest Laser line: 488 nm: Reactive Group: Isothiocyanate: Reactivity: Primary amines: Cell Permeable: Yes Excitation maximum = 495 nm; emission maximum = 515 nm; extinction coefficient = 73,000 M-1 cm-1; quantum yield = 0.92. Optical Data for TFAX 488, TFP Emission Color infrared extinction coefficients (extinction cross-section per mass) were measured.

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2 dagar sedan · Absorption and extinction coefficient theory. The velocity of propagation of a electromagnetic wave through a solid is given by the frequency-dependent complex refractive index N = n - ik where the real part, n is related to the velocity, and k, the extinction coefficient is related to the decay, or damping of the oscillation amplitude of the incident electric field.

A large number of rhodamine dyes, including Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 568, ATTO488, ATTO532, and ATTO565, were found to form stable radical anions upon irradiation in the presence of thiol compounds such as β-mercaptoethylamine (MEA).